Eukaryotic chain specificity

Service Introduced: Clear the rRNA in the tissue sample, retain mRNA and other non-coding RNA, and then build a library for sequencing of the sample, after obtaining the sequencing data, you can analyze the transcript is from the righteous or antisense DNA strand, you can accurately count the local number of transcripts and confirm the structure of the gene, and you can find more antisense transcripts, providing a data basis for subsequent analytical research. Click to download the "Sequencing Service Application Form", fill in the form as required and send it to zhangxinshuang@ cibr.ac.cn.

Sample request：

RNA Library type	Sample quality and total quantity requirements (single database construction)
Eukaryotic chain specificitys	M≥3μg, RIN≥6.8 (animals), 6.3 (plants, fungi), baseline flat (soft body, etc. without 28S species)

Library type	Sample type	Deliver guide
Chain-specific libraries	Cell	The tissue should not be less than 1-1.5g, frozen in liquid nitrogen
	Blood	The number of cells was 7-8×10⁷
	Tissue	Mammals ≥6ml
	Bacteria	About 3 90mm plates /80mL of freshly cultured thalli

Technical route:

Frequently asked questions:

Q: How do you remove rRNA?

Since rRNA is relatively conserved, we will select suitable kits for rRNA removal according to different species

Product & Services

Eukaryotic chain specificity