Eukaryotic common transcriptome

Service Introduced: Eukaryotes to specific tissues or cells of a particular state of transcription of mRNA sequencing, transcription of this information is obtained, and the reference genome compare at the same time, can fully get mRNA sequence information, further analysis of gene expression level, differences in genetic analysis and forecast new transcript, provide data basis for subsequent analysis. Click to download the "Sequencing Service Application Form", fill in the form as required and send it to zhangxinshuang@cibr.ac.cn.

Sample request：

RNA Library type	Sample quality and total quantity requirements (single database construction)
Ordinary transcriptomes	M≥3μg, RIN≥6.8 (animals), 6.3 (plants, fungi), baseline flat (soft body, etc. without 28S species)

Library type	Sample type	Deliver guide
Ordinary transcriptomes	Cell	The tissue should not be less than 1-1.5g, frozen in liquid nitrogen
	Blood	The number of cells was 7-8×10⁷
	Tissue	Mammals ≥6ml
	Bacteria	About 3 90mm plates /80mL of freshly cultured thalli

Technical route:

Frequently asked questions:

Q: How large is the amount of general sequencing data in a common transcriptome?

6G clean data is generally recommended for reference genomes, and 10-12G clean data is recommended for samples that want to detect low-abundance transcripts

Product & Services

Eukaryotic common transcriptome