Regular DNA small fragment sequencing

Service Introduction: Regular DNA fragments are 350bp small fragment libraries that meet the requirements of computer sequencing, and a large amount of genetic information can be obtained by sequencing small fragment libraries based on whole genome sequencing through high-throughput sequencing technology. Such as single nucleotide polymorphism (SNP), insertion deletion (InDel), structural variation (SV), copy number variation (CNV) and other genetic polymorphism data, thus laying the data foundation for subsequent analysis. Click to download the "Sequencing Service Application Form", fill in the form as required and send it to zhangxinshuang@cibr.ac.cn.

Sample request：

Sample type	Deliver guide
Tissue	The tissue should not be less than 0.5g and be frozen with liquid nitrogen
Cell	The number of cells is not less than 2.0×107
Animal blood	For mammals, not less than 1.5ml
Saliva	Not less than 6ml
FFPE	No less than 15 patches, rolls, tissue ≥0.5g

Technical route:

Frequently asked questions

Q: What genetic variants can be detected by resequencing?

Genetic variants that can currently be detected by resequencing include SNPs, Indels, SVs, CNVs, and more.

Q: What is the depth of resequencing?

General individual weight sequencing, 10-30X sequencing depth is recommended, and population resequencing is recommended 5-10X. However, when the genome is more complex and the accuracy of SNP is relatively high, the sequencing depth needs to be further improved.