Hi-C sequencing
Service Introduced: This technology is a chromosome conformation capture technology, which studies the relationship between the entire genome-wide chromatin DNA in the spatial position, and captures the interaction mode of all DNA in the chromatin, which can obtain high-resolution chromatin three-dimensional structure information, which can not only study the interaction between chromosome fragments, but also be used for genome assembly, haplotype mapping construction, etc., to provide a data basis for subsequent analysis. Click to download the "Sequencing Service Application Form", fill in the form as required and send it to zhangxinshuang@cibr.ac.cn.
Sample request：
|
Library type |
Sample type |
Deliver guide |
|
HIC |
Tissue |
Tissue is not less than 1.5 g; Adipose tissue is not less than 4 g, liquid nitrogen is quick-frozen |
|
Cell |
The cell volume ≥ 1.0×107/time, it is recommended to perform mycoplasma identification before sending samples and send fixed cell lines |
Technical route:
Frequently asked questions:
Q: Does Hi-C need to be repeated?
It is generally recommended to do 3 repetitions, considering the cost, you can do 2 repetitions
Q: What is the general sequencing depth?
It is recommended to sequence 100X, the higher the depth, the higher the resolution
Tel：
Email:genomics@cibr.ac.cn
Fax：
Address：Beijing brain science and brain like research center, No.26 Science Park, Zhongguancun Life Science Park, Changping District, Beijing
Web:http://www.cibr.ac.cn
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