ATAC

Service Introduced: It is a method of analyzing transposase's accessibility nuclear chromatin by high-throughput sequencing, cutting the open nuclear chromatin region in a specific space-time by Tn5 transposase, and then obtaining the regulatory sequence of active transcription in the genome in this space-time, and then inferring the accessibility of the chromatin region through high-throughput sequencing technology, providing a data basis for subsequent analysis. Click to download the "Sequencing Service Application Form", fill in the form as required and send it to zhangxinshuang @cibr.ac.cn.

Sample request：

Technical route:

Frequently asked questions:

Q: Does ATAC-seq need to set repeats?

It is recommended to set 3 repetitions for more accurate results

Q: What kind of samples is ATAC suitable for?

ATAC-seq is suitable for a variety of cell or tissue samples with reference genomic species. However, the processing conditions of different species and samples vary greatly, and the construction of stockpiles of many species is at certain risk

Q: What are the similarities and differences between atAC-seq's method of studying transcription factor binding sites and ChIP-seq?

Different from the method of ChIP-seq directly obtaining protein binding sites, ATAC-seq is mainly based on the sequence of mold bodies (motif) genes in the open region to predict the transcription factors that may bind, which is suitable for some transcription factor studies that lack ChIP-level antibodies or are difficult to do ChIP enrichment.