smart-seq

Service Introduction: Full-length RNA was synthesized and amplified by reverse transcription using SMARTscribeRT enzyme, which could cover the entire transcript and greatly increase the number of gene detections. On the basis of expression analysis, further studies such as variable shear and SNV detection can be carried out, and intracellular gene expression and differences can also be studied from the single cell level. Click to download the "Sequencing Service Application Form", fill in the form as required and send it to zhangxinshuang@cibr.ac.cn.

Sample request：

Library type	Sample type	Deliver guide
Smart-seq	Cell	Cell count: 1-500 (cell lysate + cells should not exceed 3.5ul)
	Blood	Sufficient amount of blood is drawn and added to the anticoagulant tube containing heparin sodium, which can be sent in a short time. After obtaining the blood, single-cell suspension is prepared, and 1-500 cells are sorted according to the requirements (the amount of blood will be adjusted according to the experimental purpose)..
	Tissue	Sufficient amount of fresh tissue can be sent in a short time. After obtaining the tissue, single-cell suspension can be prepared, and 1-500 cells can be sorted according to requirements (the amount of tissue varies according to the purpose of the experiment).

Technical route:

Frequently asked questions:

Q: There are small fragments when the single-cell transcriptome is expanded, why?

Possible causes are as follows:

1. There is degradation in the sample;

2. There are inhibitors in the sample, such as: pancreatin, trypan blue, high concentration of EDTA, etc

3. Excessive endogenous RNase;

Q: What is the size of the CDNA amplified by Smart-seq generally?

Generally around 2000bp

Product & Services

smart-seq