PCR free sequencing

Service Introduction: PCR-free library is a library that can be used for high-throughput sequencing without any PCR during the whole library construction process, which can improve the detection rate and accuracy of rare mutations and lay a good data foundation for low-abundance mutations. Click to download the "Sequencing Service Application Form", fill in the form as required and send it to zhangxinshuang@cibr.ac.cn.

Sample request：

PCR – free library type	Total sample quantity requirement (single database construction)
PCR – free library	M≥10μg
＜500bp PCR product (single band)	Separate library: M≥3μg; Mixing sample construction library: M ≥0.5μg
Genomic DNA / > 500bp PCR product	M≥6μg
Amplicon	M≥0.5μg

Sample type	PCR-free
Animal tissue	≥3g
Animal blood	≥10ml

Technical route:

Frequently asked questions

Q: What types of samples are suitable for PCR-free libraries?

Some samples with complex secondary structures, high GCs, and PCR preferences can be built with a PCR-free library.

Q: What is the fragment size of the PCR-free library?

PCR-free library itself has no special requirements for fragment size, for some PCR product libraries according to the size of PCR products to build libraries, for genome libraries is recommended or around 350bp, so that the data quality of the library is relatively good.

Product & Services

PCR free sequencing