Human exon sequencing

Service Introduction: Exome sequencing is a technique that uses probes to capture DNA sequences enriched in exon regions and discover genetic mutations associated with protein functional variations through high-throughput sequencing. Although the exon region accounts for less than 2% of the whole genome, the high depth of sequencing facilitates the discovery of mutations and provides data basis for subsequent analysis. Click to download the "Sequencing Service Application Form", fill in the form as required and send it to zhangxinshuang@cibr.ac.cn.

Sample request：

DNA library type	Total sample quantity requirement (single database construction)
Exon library	M≥1.5μg
Target region library	M≥1.5μg

Sample type	Deliver guide
Tissue	≥0.5g
Cell	The number of cells is not less than 5.0×107
Animal blood	For mammals, not less than 2 ml
Saliva	Not less than 6ml
FFPE	No less than 15 patches, rolls, tissue ≥0.8g

Technical route:

Frequently asked questions：

Q: How do I choose the right clip size?

The general small fragment library selection of 350bp to meet the analysis needs, due to the timing PE150 sequencing method we use, the fragment will be measured through, resulting in some data waste, but for the need for overlap data, it is recommended to choose a fragment size of 250bp.

Q: Does building libraries of different fragment sizes have an impact on subsequent data analysis?

If there is no special analysis needs, there is generally no impact, but the size of the fragment will have an impact on the data quality, the fragment of small data will cause data waste, the quality of the partial big data will be poor and the output is unstable, it is generally recommended to choose a fragment of about 350bp.

Product & Services

Human exon sequencing