DNA unconventional fragment sequencing

Service Introduction: DNA unconventional small fragments are non-350bp small fragment libraries that meet the requirements of computer sequencing (generally, the fragments of constructed libraries are distributed in 250bp, 300bp, 400bp and 500bp), and a large amount of genetic information can be obtained by sequencing small fragment libraries based on whole genome sequencing through high-throughput sequencing technology. It can be used to obtain single nucleotide polymorphism (SNP), insertion deletion (InDel), structural variation (SV), copy number variation (CNV) and other genetic polymorphism data. It can also be used to evaluate genome size, heterozygosity, GC content and other information, and lay a data foundation for subsequent analysis. Click to download the "Sequencing Service Application Form", fill in the form as required and send it to zhangxinshuang@cibr.ac.cn.

Sample request：

Technical route:

Frequently asked questions：

Q: How do I choose the right clip size?

The general small fragment library selection of 350bp to meet the analysis needs, due to the timing PE150 sequencing method we use, the fragment will be measured through, resulting in some data waste, but for the need for overlap data, it is recommended to choose a fragment size of 250bp.

Q: Does building libraries of different fragment sizes have an impact on subsequent data analysis?

 If there is no special analysis needs, there is generally no impact, but the size of the fragment will have an impact on the data quality, the fragment of small data will cause data waste, the quality of the partial big data will be poor and the output is unstable, it is generally recommended to choose a fragment of about 350bp.